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FLASH is an advanced transient absorption curve fitting software complementary to the L900 package. Fitting complex kinetic decays only takes a few seconds with FLASH.
The program combines a user-friendly graphical interface with an advanced least-square minimisation algorithm fitting to models for 1st, 2nd, and combined 1st and 2nd order kinetics. Traces can be fitted simultaneously with linked parameters using global analysis.
FLASH is directly compatible with L900 data as well common formats such as ASCII. Results can be exported in a variety of text and image formats for ease of publication.
Analysis options include:
A set of TA data (in this case 2nd order kinetic traces) can be fitted and displayed simultaneously in FLASH.
Global analysis allows linking parameters across the full set of TA traces to analyse.
Advanced Analysis Tools of FLASH |
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Analysis of first order kinetics, single curve analysis and multiple curve batch analysis, with up to four exponential terms |
Global analysis of first order kinetics, including generation of lifetime discriminated spectra or decay associated spectra (DAS) |
Analysis of second order kinetics, single curve Analysis and multiple curve batch analysis |
Global Analysis of second order kinetics, including generation of time resolved spectra |
Analysis of mixed first and second order kinetics, single curve analysis and multiple curve batch analysis |
Global Analysis of mixed first and second order kinetics, including generation of time resolved spectra |
Calculation of parameter confidence intervals by support plane analysis |
The FLASH software licence is protected by a USB key |
Specifications | Spectral | Phosphorescence Lifetime | Fluorescence Lifetime |
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Mode of Operation | Single Photon Counting | Time-Resolved Single Photon Counting (Multi Channel Scaling - MCS) | Time-Correlated Single Photon Counting (TCSPC) |
Lifetime range | Milliseconds to hours | 10 ns - 50 s * | 5 ps - 10 µs * |
Sensitivity | >35,000:1 ** | n/a | n/a |
Excitation Sources | |||
Type | 450 W ozone-free xenon arc lamp | Microsecond flashlamp | Picosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs) |
Spectral Range | 230 nm- > 1000 nm | 200 nm - >1000 nm | Discrete wavelengths between 250 nm - 980 nm |
Pulse width | n/a | 1 µs - 2 µs | from 60 ps |
Options | Ozone generating lamp with spectral range 200 nm - >1000 nm | Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDs | Nanosecond flashlamp 200 nm - >400 nm pulse width <1 ns |
* source and detector dependent ** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method |
Monochromator | Specifications |
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Type | Czerny-Turner with 'plug and play' triple grating turret |
Focal Length | 325 mm (double monochromators: 2 x 325 mm) |
Stray Light Rejection | 1:10-5 (single), 1:10-10 (double) |
Gratings | Mounted to triple grating turret |
Accuracy | +/- 0.2 nm * |
Minimum Step Size | 0.01 nm * |
Option | Spectrographs available for operations of CCDs and diode array detectors |
* grating dependent |
Detectors | PMT-900 | PMT-1010 | PMT- 1400/1700 | HS-PMT | MCP-PMT | HS-HPD |
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Spectral Range | <200 nm - 870 nm | <200 nm - 1010 nm | 500 nm - 1700 nm | <230 nm - 850 nm | <200 nm - 850 nm | 220 nm - 870 nm |
Dark Count Rate | <50 cps (-20 °C) | <200 cps (-20 °C) | <20 kcps or <200 kps | <150 cps (0 °C) | <10 cps (-20 °C) | <200 cps (10 °C) |
Reponse Width | 600 ps | 800 ps | 400 ps or 800 ps | 180 ps | <25 ps | 20 ps |
Options | A wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm |