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FAST

Advanced Fluorescence Lifetime Analysis Software

FAST is Edinburgh Instruments’ proprietary software for lifetime decay analysis stretching beyond the standard TCSPC applications.

FAST

Advanced Fluorescence Lifetime Analysis Software

  • Advanced applications: Anisotropy, Förster kinetics, micellar quenching, and more
  • Up to 200 lifetimes: simultaneously fitted with global analysis
  • Bespoke package: Exclusive algorithms owned and developed by Edinburgh Instruments
  • Robust and reliable: with guaranteed fit results

   Download the latest trial version of FAST Software

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PRODUCT DESCRIPTION

FAST (Fluorescence Analysis Software Technology) offers advanced analysis of photoluminescence decay kinetics with outstanding robustness, speed, and fits that are 100% convergent.

FAST contains a library of advanced data reconvolution and curve fitting routines based on proprietary data processing algorithms, which in both speed and reliability surpass the conventional Marquardt-Levenberg algorithm.

Despite the sophisticated and challenging analysis models, FAST is easy to operate, with an intuitive and user-friendly interface. A wide range of data input, on-screen visualisation, and export facilities are available.

Experimental data can be analysed one at a time or in batch mode. The latter is particularly useful for larger data sets such as in assay development and screening.

PRODUCT IMAGE

Software Features

Global Multi-Exponential Analysis

The Global Analysis Sub-Routine allows one to test decay models for common (linked) lifetime parameters.

Discrete Component Analysis

The GLSA (Global Least Square Analysis) algorithm is 100% convergent with either automatic choice of initial values or manual parameter control.

Stretched Exponential Analysis

FAST offers the fitting of fluorescence decays with up to four stretched exponential terms (Kohlrausch Quenching Model). Shift and background may be left as fitting parameters, or may be fixed.

Lifetime Distribution Analysis

Exponential series method combined with NTSVD (Non-Negative Truncated Singular Value Decomposition) technology. The algorithm does not require predefined shapes of the distributions.

Förster Kinetics

A fitting routine based on GLSA for the analysis of Förster kinetics between donors and randomly (homogeneously) distributed acceptors is included.

Micellar Quenching

Fluorescence kinetics of Fluorophores embedded in micelles can often be described by the Micellar Quenching model included in FAST.

Anisotropy

Time-resolved fluorescence anisotropy models include a spherical rotor, two spherical rotors, spherical rotor with restricted rotation and ellipsoidal rotor.

TECHNICAL SPECIFICATIONS

Technical Specifications
Advanced Analysis Tools of FAST
Lifetime distribution analysis
Exponential component analysis
Support plane analysis for the calculation of lifetime confidence intervals
Global exponential component analysis
Stretched exponential components analysis
Förster kinetics analysis
Micellar quenching kinetics analysis
Analysis of time resolved fluorescence anisotropy kinetics
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SpecificationsSpectralPhosphorescence LifetimeFluorescence Lifetime
Mode of OperationSingle Photon CountingTime-Resolved Single Photon Counting (Multi Channel Scaling - MCS)Time-Correlated Single Photon Counting (TCSPC)
Lifetime rangeMilliseconds to hours10 ns - 50 s *5 ps - 10 µs *
Sensitivity>35,000:1 **n/an/a
Excitation Sources
Type450 W ozone-free xenon arc lampMicrosecond flashlampPicosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs)
Spectral Range230 nm- > 1000 nm200 nm - >1000 nmDiscrete wavelengths between 250 nm - 980 nm
Pulse widthn/a1 µs - 2 µsfrom 60 ps
OptionsOzone generating lamp with spectral range
200 nm - >1000 nm		Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDsNanosecond flashlamp
200 nm - >400 nm
pulse width <1 ns
* source and detector dependent
** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method
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MonochromatorSpecifications
TypeCzerny-Turner with 'plug and play' triple grating turret
Focal Length325 mm (double monochromators: 2 x 325 mm)
Stray Light Rejection1:10-5 (single), 1:10-10 (double)
GratingsMounted to triple grating turret
Accuracy+/- 0.2 nm *
Minimum Step Size0.01 nm *
OptionSpectrographs available for operations of CCDs and diode array detectors
* grating dependent
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DetectorsPMT-900PMT-1010PMT- 1400/1700HS-PMTMCP-PMTHS-HPD
Spectral Range<200 nm - 870 nm<200 nm - 1010 nm500 nm - 1700 nm<230 nm - 850 nm<200 nm - 850 nm220 nm - 870 nm
Dark Count Rate<50 cps (-20 °C)<200 cps (-20 °C)<20 kcps or <200 kps<150 cps (0 °C)<10 cps (-20 °C)<200 cps (10 °C)
Reponse Width600 ps800 ps400 ps or 800 ps180 ps<25 ps20 ps
OptionsA wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm
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