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| Compound [a] | Solvent | τ (ns) [b] | λex (nm) | λem (nm ) |
|---|---|---|---|---|
| 9-cyanoanthracene | methanol cyclohexane | 16 ±1 12.7 ± 0.7 | 295-360 295-360 | 400-480 400-450 |
| anthracene | methanol cyclohexane | 5.1 ± 0.3 5.3 ± 0.1 | 295-360 295-360 | 375-442 375-442 |
| coumarin 153 | methanol | 4.3 ± 0.2 | 295-442 | 495-550 |
| DPA | methanol cyclohexane | 8.7 ± 0.5 7.5 ± 0.4 | 295-360 295-360 | 400-475 400-475 |
| erythrosin B | water methanol | 0.089 ± 0.003 0.47 ± 0.02 | 488-568 488-568 | 550-580 550-590 |
| NATA | water | 3.1 ± 0.1 | 295-309 | 330-410 |
| N-methylcarbazole | cyclohexane | 14.1 ± 0.9 | 290-325 | 350-400 |
| POPOP | cyclohexane | 1.12 ± 0.04 | 295-360 | 380-450 |
| PPO | methanol cyclohexane | 1.65 ± 0.05 1.36 ± 0.04 | 295-330 290-325 | 340-400 360-450 |
| p-terphenyl | methanol cyclohexane | 1.17 ± 0.08 0.98 ± 0.03 | 284-315 290-315 | 330-380 330-390 |
| rhodamine B | water methanol | 1.74 ± 0.02 2.5 ± 0.1 | 488-575 295,488-568 | 560-630 550-630 |
| rubrene | methanol | 9.9 ± 0.3 | 300, 488, 514 | 550-610 |
| SPA | water | 31.2 ± 0.4 | 300-330 | 466-520 |
Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single up to 5 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves for concentration measurements.
Time Scan: Perform kinetic measurements for time periods ranging from 10 seconds to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.
Wavelength scan: Perform a full spectral scan from 190 nm – 1100 nm at any of 9 incremental and preset selectable scan speeds starting from a high resolution 10 nm/min up to a maximum scan speed of 6,000 nm/min. Data is displayed as a graphical spectrum on which data analysis can be performed through the touchscreen interface with zoom, peak-valley, smoothing and other functions available through the easy to use menu interface.
| Detector | PMT-1400-TE | PMT-1400-TE | PMT-1700 | PMT-1700-TE | InGaAs-1650 | InGaAs-2050 | InGaAs-2550 |
|---|---|---|---|---|---|---|---|
| PMT-900 | 500 nm – 870 nm | x | 500 nm – 870 nm | x | x | x | x |
| PMT-980 | 500 nm – 980 nm | x | 500 nm – 980 nm | x | 870 nm – 980 nm | 900 nm – 980 nm | 900 nm – 980 nm |
| PMT-1010 | 500 nm – 1010 nm | 950 nm – 1010 nm | 950 nm – 1010 nm | 950 nm – 1010 nm | 870 nm – 1010 nm | 900 nm – 1010 nm | 900 nm – 1010 nm |
To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the DS5 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can all be validated.
