KNOWLEDGEBASE
Search
Close this search box.
KNOWLEDGEBASE
Search
Close this search box.
Edit Content
  1. Home
  3. Product
  5. FS5 Spectrofluorometer

FS5

Spectrofluorometer

Compact high-sensitivity spectrofluorometer adaptable to a wide range of research and analytical applications.

FS5

Spectrofluorometer

  • High Sensitivity: Photon counting detection, with a signal-to-noise ratio of 10,000:1, provides exceptional sensitivity for the detection of weak fluorescence signals
  • 2-in-1 Spectrometer: Measure both absorption and fluorescence spectra in a single instrument
  • Adaptable: Tailor to your requirements, from a dedicated spectral fluorometer to an all-in-one characterisation platform with time-resolved fluorescence and quantum yield
  • Plug & Play Sample Modules: Auto-recognised sample modules that can be swapped in seconds, enabling a rapid transition between different sample formats and measurement types
  • Near-Infrared Detection: Extend the measurement range out to 1700 nm by adding a NIR detector, ideal for studying NIR-emitters, such as quantum dots and upconversion nanoparticles
  • Automated Acquisition: Maximise your lab productivity with the FS5 Fluoracle software Batch feature to create automated sample measurement sequences
App Notes / Downloads

Live Video Demonstration

Send us your samples, and we can measure them live in an online demonstration

Contact us to arrange an online demonstration

Click Here to complete an enquiry form for this product...

PRODUCT DESCRIPTION

The FS5 is a high-performance, fully integrated spectrofluorometer engineered to meet the most stringent demands of research and analytical applications.

Equipped with top-tier optical components, photon-counting sensitivity, and fluorescence lifetime capabilities, the FS5 excels in both rapid routine analysis and sensitive research tasks.

This compact benchtop instrument adapts seamlessly to your needs, offering flexibility with multiple source and detector configurations without expanding its footprint. As standard, the FS5 includes a photon-counting PMT detector for visible-range fluorescence spectra and an absorption detector. It can be easily upgraded to perform NIR measurements up to 1650 nm, time-resolved fluorescence or phosphorescence, photoluminescence quantum yields, and anisotropy.

PRODUCT VIDEO

PRODUCT DETAILS

SOFTWARE

Fluoracle® is the operating software for the FS5 Spectrofluorometer. It controls all FS5 steady state and time-resolved spectrometer features with a straightforward design concept: focus on all modern photoluminescence spectroscopy applications and provide a user-friendly interface with ‘ready to publish’ outputs. 

Figure 1: Fluoracle software interface

Whether you select a basic spectral scanning version of FS5, or you go for an advanced version that includes TCSPC lifetime measurements or integrating sphere measurements, the all-inclusive software will provide instrument options automatically, from data acquisition to analysis and presentation. 

Upgrade to FAST Software for advanced fluorescence lifetime analysis. 

MEASUREMENT EXAMPLES

Steady State
Quantum Dot Emission with NIR Detector 

The FS5 Spectrofluorometer can measure emission spectra up to 1650 nm when equipped with an NIR PMT or InGaAs detector. These upgrades maintain the standard photon-counting PMT for high sensitivity in the UV-Visible range. 

Absorption and emission spectrum of PbS quantum dots

Figure 1: Absorption and emission spectrum of PbS quantum dots in tetrachloroethylene measured in an FS5 with InGaAs-1650 NIR detector (NIRA+ upgrade).

Kinetic Scans with Stopped Flow 

Kinetic absorption and emission scans are available in the standard FS5 spectrofluorometer. This capability can be expanded further with a stopped flow accessory for rapid injection of reactants. 

Figure 2: Bleaching of K4[Fe(CN)6] by Ascorbic Acid using the stopped-flow accessory. The absorption kinetic of Fe(CN)63- bleaching was recorded using the transmission detector of the FS5.

High Throughput Measurements with Microplate Reader 

The SC-41 plate reader module allows measuring liquid samples with microplates up to 384 wells. As with all sample holder accessories, this microplate reader is compatible with FS5 upgrade options such as NIR detection and time-resolved photoluminescence. 

Live measurement display for the SC-41 plate reader

Figure 3: Live measurement display for the SC-41 plate reader

Spectral Anisotropy Measurements 

If the FS5 is equipped with the polariser (POL) upgrade, automated measurements of fluorescence anisotropy are possible. A software wizard in Fluoracle guides the user through the acquisition and analysis of spectral and time-resolved (if equipped with TCSPC) anisotropy. 

 Fluorescence Anisotropy measurement of Rhodamine 6G in glycerol at room temperature

 Fluorescence Anisotropy measurement of Rhodamine 6G in glycerol anisotropy results

Figure 4: Fluorescence Anisotropy measurement of Rhodamine 6G in glycerol at room temperature (top) and anisotropy result (bottom). 

Trend Analysis 

Fluoracle features user-friendly wizards for acquiring and fitting calibration curves, perfect for developing quantitative analysis methods. Peak signals, areas, and ratios can be fitted to linear and non-linear curves. 

Screenshot of Ratio trend analysis using Fluoracle

Figure 5: Ratio trend analysis using Fluoracle.

Time-Resolved - TCSPC
Fluorescence Lifetime Decays with Temperature Dependence 

The SC-25 TE cooled sample cassette is controlled by the Fluoracle software allowing automated temperature-dependent measurements of spectra and/or lifetimes. In the example below, it is used for Time-Correlated Single Photon Counting (TCSPC) as a function of temperature. 

Automatically acquired temperature lifetime map of Rhodamine-B emission in H2O measured using the SC-25 TE-cooled module, from +10 °C to +80 °C  

Figure 1: Automatically acquired temperature lifetime map of Rhodamine-B emission in H2O measured using the SC-25 TE-cooled module, from +10 °C to +80 °C.

Time-Resolved - MCS
Upconversion Photoluminescence Lifetime 

The FS5 can be equipped with a Multichannel Scaling (MCS) upgrade and a range of high-power lasers suitable for upconversion measurements. Lasers at 808 nm and 980 nm are particularly useful for upconversion of lanthanide samples and can be operated in CW mode for spectra and pulsed mode for MCS lifetimes. 

 Photoluminescence decay of upconversion nanoparticles (UCNPs) in water

Figure 1: Photoluminescence decay of upconversion nanoparticles (UCNPs) in water at 653 nm acquired in an FS5 with MCS, using a 2 W 980 nm laser diode in pulsed mode at 500 Hz.

PLQY

Absolute photoluminescence quantum yield (PLQY) is available for the visible and NIR range using the SC-30 integrating sphere module. An intuitive wizard in the Fluoracle software calculates the PLQY automatically for different sample configurations. 

Absolute PLQY measurement from a Tb3+ complex in DI water in the FS5 with SC-30 sample cassette

Figure 1: Absolute PLQY measurement from a Tb3+ complex in DI water in the FS5 with SC-30 sample cassette (PLQY = 29.01%). The blank measurement was performed in DI water.

 

Customer Testimonials

“During the process of contacting sales reps from multiple companies, the feedback I received from Edinburgh was far superior than the other companies. This, alongside the applicability of the instrument to our work, was a huge selling point for purchasing from Edinburgh. After-sales support has also been superb, as we look to add more functionality to the instrument through accessories and/or upgrades.”
FS5 Customer Testimonial
Customer Testimonial
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
Name 01
Title

Options and Upgrades

Spectral Range Upgrades

A range of upgrades for NIR spectral coverage set the FS5 apart from other benchtop spectrofluorometers. A second detector is fitted to expand the operating spectral range without sacrificing the performance in the standard range. There are several versions available: 

FS5-NIR is fitted with an extra cooled side-window PMT and grating optimized for sensitivity up to 1010 nm. 

FS5-NIR+ adds a TE-cooled NIR PMT and grating for photon-counting sensitivity up to 1650 nm. 

FS5-NIRA+ features an additional analogue NIR detector and grating for spectral coverage up to 1650 nm (steady-state measurements only). 

Time-Resolved Measurements

Both Time-Correlated Single Photon Counting (TCSPC) and Multichannel Scaling (MCS) are available as upgrades to the FS5 Spectrofluorometer without compromising its spectral capabilities. 

FS5 – MCS: The instrument has all the features of the standard FS5, plus the capability to measure sample decays with lifetimes of over 1 μs (phosphorescence, lanthanide decays). The changeover between the standard continuous xenon light source and the pulsed xenon flash lamp is software controlled and the acquisition mode automatically changes from standard to time-resolved photon counting. The software incorporates fitting and reconvolution analysis for lifetime evaluation. The instrumental response function (IRF) of this instrument is 2 μs. Lifetimes from <5 μs to >10 s can be accurately measured. 

FS5 – TCSPC: The instrument has all the features of the standard FS5, plus the capability of fluorescence lifetime measurements in the picosecond, nanosecond and the lower microsecond (<50 μs) time range. The FS5-TCSPC version requires picosecond pulsed diode lasers and LEDs for excitation, which are simply attached to the FS5-TCSPC housing and are compatible to all sample holder options. The software offers numerical reconvolution and curve fitting. TCSPC lifetime measurements with the standard FS5 detector will have an instrumental response function (IRF) of approximately 800 ps (FS5-TCSPC), enabling <100 ps lifetimes to be resolved (source dependent). 

For challenging applications, the IRF can be improved by fitting a second, faster detector (FS5-TCSPC+). Using an EPL as an excitation source in this configuration will result in an IRF of <200 ps, enabling approximately 25 ps lifetimes to be resolved depending on the source used. 

Polarisation Upgrade

The FS5-POL upgrade equips the FS5 with fully computerised polarisers in both excitation and emission arms. Automated measurements and generation of anisotropy curves, both raw and G-factor corrected, are supported by the Fluoracle software. If combined with the TCSPC option, time-resolved fluorescence anisotropy measurements and analysis will also be possible. 

Sample Holders / Cassette Upgrades

The FS5 offers measurement modules available for practically all applications. These can be purchased at any time and can be easily installed by the user. The installation takes no more than a few seconds. The Fluoracle software automatically recognises which of the measurement modules is in use. This reduces the complexity in the user interface and ensures ease of operation. 

Sample Cassettes (SC) available include: 

SC-05: Standard Cuvette Holder 

SC-06: Two-position Cuvette Holder. Offers automated absorption and two-position measurements. 

SC-10: Solid Sample Holder (upright/tilted sample). With external position control. 

SC-15: Solid Sample Holder (horizontal sample) 

SC-20: Thermostatic Sample Holder (external water bath regulation) 

SC-24: TE-Cooled Sample Holder (-50°C to +150°C), software controlled 

SC-25: TE-Cooled Sample Holder (-35°C to +105°C), software controlled 

SC-26: TE-Cooled Sample Holder (-35°C to +150°C), software controlled 

SC-27: 4-position TE-Cooled Sample Holder (-40°C to +105°C), software controlled including automated sample exchange 

SC-28: Heated Sample Holder for Powders (up to 300°C) 

SC-30: Integrating Sphere for absolute PLQY 

SC-41: Microplate reader (up to 384 wells), for automated multiple measurements of spectra and/or lifetimes 

SC-50: Optical Fibre Launcher (liquid light guides, SMA, FC) 

SC-70: Liquid Nitrogen EPR Dewar 

SC-80: Liquid Nitrogen Cryostat, software controlled 

Sample Cassette Holders

Contact us directly to customise your sample cassette

MicroPL Upgrade

The MicroPL upgrade allows spectral and time-resolved photoluminescence measurements of samples in the microscopic scale. The FS5 is upgraded with a microscope so you can finely tune both the excitation light (illumination) and the detected emission, using widefield or point excitation. 

The microscope can be supplied as either an upright or inverted microscope. Imaging cameras are available spanning the spectrum from the visible to the near-infrared, up to 1700 nm. Excitation can be provided by halogen lamps (widefield excitation), picosecond pulsed diode lasers (EPL Series, HPL Series) and pulsed LEDs (EPLED Series), supercontinuum sources and Nd:YAG lasers (lasers provide point source illumination). Steady state emission spectra and fluorescence lifetime measurements can be obtained from specific spots on your sample, when using appropriate lasers. Lasers can achieve a spot size of ~2 μm (objective dependent). 

The optional FLIM add-on includes a computer-controlled XYZ stage and unlocks special features in the Fluoracle software including advanced analysis options for maps, such as multi-component decay fitting algorithms. 

FS5 with MicroPL attachment

Download the MicroPL datasheet for full details.  

TECHNICAL SPECIFICATIONS

Specifications
Specification
OpticsAll-reflective for a wavelength independent focus with high brightness (small focus) at the sample
Source150 W CW Ozone-free xenon arc lamp (UV-enhanced ozone generating option available)
MonochromatorsCzerny-Turner design with dual grating turret; plane gratings for accurate focus at all wavelength and minimum stray light
Spectral Coverage - Excitation<230 nm - 1000 nm
Spectral Coverage - Emission200 nm - >870 nm
Filter WheelsFully automated; included in both the excitation and emission monochromators
Bandpass - Excitation/Emission0 to 30 nm, continuously adjustable
Wavelength Accuracy - Excitation/Emission± 0.5 nm
Scan Speed - Excitation/Emission100 nm/s
Integration Time1 ms - 200 s
Emission DetectorPhotomultiplier, spectral coverage 200 nm - 870 nm, cooled and stabilised
Reference DetectorUV enhanced silicon photodiode
Transmission DetectorUV enhanced silicon photodiode
Signal-to-Noise Ratio of Water Raman SignalSNR SQRT >10,000:1
Dimensions104 cm (w) x 59 cm (d) x 32 cm (h)
Weight55 kg
* standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method
Emission Wavelength Extension
ModelPMT-EXTFS5-NIRFS5-NIR+FS5-NIRA+
Emission coverage200 nm - >980 nm200 nm - >870 nm plus 200 nm – 1010 nm200 nm - >870 nm plus 950 nm - >1650 nm200 nm - >870 nm plus 870 nm - >1650 nm
NotesPMT-980 replaces standard PMT-900PMT-EXT recommended for complete spectral coverageSpectral measurements only (no lifetime)
Lifetime Upgrades
ModelFS5-MCSFS5-TCSPCFS5-TCSPC+
SourcesMicrosecond Xenon flashlamp, Picosecond pulsed diode lasers (EPL Series), Picosecond pulsed LEDs (EPLED Series), Variable pulse sources (VPL/VPLED Series)Picosecond pulsed diode lasers (EPL Series), Picosecond pulsed LEDs (EPLED Series)Picosecond pulsed diode lasers (EPL Series), Picosecond pulsed LEDs (EPLED Series)
Lifetime Range< 5 µs -> 10 s90 ps* - > 10 µs < 25 ps* - 10 µs
*Source dependent
Edit Content
SpecificationsSpectralPhosphorescence LifetimeFluorescence Lifetime
Mode of OperationSingle Photon CountingTime-Resolved Single Photon Counting (Multi Channel Scaling - MCS)Time-Correlated Single Photon Counting (TCSPC)
Lifetime rangeMilliseconds to hours10 ns - 50 s *5 ps - 10 µs *
Sensitivity>35,000:1 **n/an/a
Excitation Sources
Type450 W ozone-free xenon arc lampMicrosecond flashlampPicosecond pulsed diode lasers (EPLs, HPLs) and pulsed LEDs (EPLEDs)
Spectral Range230 nm- > 1000 nm200 nm - >1000 nmDiscrete wavelengths between 250 nm - 980 nm
Pulse widthn/a1 µs - 2 µsfrom 60 ps
OptionsOzone generating lamp with spectral range
200 nm - >1000 nm		Low to medium repetition rate pulsed lasers. Variable pulse width VPL and VPLEDsNanosecond flashlamp
200 nm - >400 nm
pulse width <1 ns
* source and detector dependent
** standard water Raman measurement conditions: Excitation wavelength = 350 nm, Excitation and emission bandwidths = 5 nm, step size = 1 nm, integration time = 1 s, emission wavelength = 397 nm, noise measured at 450 nm and calculation based on the SQRT method
Edit Content
MonochromatorSpecifications
TypeCzerny-Turner with 'plug and play' triple grating turret
Focal Length325 mm (double monochromators: 2 x 325 mm)
Stray Light Rejection1:10-5 (single), 1:10-10 (double)
GratingsMounted to triple grating turret
Accuracy+/- 0.2 nm *
Minimum Step Size0.01 nm *
OptionSpectrographs available for operations of CCDs and diode array detectors
* grating dependent
Edit Content
DetectorsPMT-900PMT-1010PMT- 1400/1700HS-PMTMCP-PMTHS-HPD
Spectral Range<200 nm - 870 nm<200 nm - 1010 nm500 nm - 1700 nm<230 nm - 850 nm<200 nm - 850 nm220 nm - 870 nm
Dark Count Rate<50 cps (-20 °C)<200 cps (-20 °C)<20 kcps or <200 kps<150 cps (0 °C)<10 cps (-20 °C)<200 cps (10 °C)
Reponse Width600 ps800 ps400 ps or 800 ps180 ps<25 ps20 ps
OptionsA wide variety of other photomultipliers and analogue detectors are available up to 5,500 nm

PRODUCT BROCHURE

Enquire Now

Keep up to date with the latest from Edinburgh Instruments

Join our mailing list today and be in with a chance to win £50 Amazon voucher!

LOCATION:
ABOUT:
PRODUCTS:
LEGALS:
SOCIALS:
Youtube Linkedin X-twitter Facebook
©2024 Edinburgh Instruments. Registered in England and Wales No: 962331. VAT No: GB 271 7379 37
Skip to content