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MicroPL is coupled to the spectrometer via liquid light guides or fibres. It is possible to excite the full field of view of the microscope’s objective (widefield excitation), or a specific point in the sample with a laser (point excitation).
The spectrometer software selects the wavelength and bandwidth of the light exciting the sample. This provides much more flexibility than traditional fluorescence microscopy and removes the need for excitation filters.
Point excitation can be performed with either CW or pulsed lasers for spectral or lifetime PL measurements. MicroPL is compatible with Edinburgh Instruments EPL, HPL, and VPL diode lasers. A spot size of ~2 μm (source and objective dependent) is achievable, enabling to resolve PL spectra and lifetimes in the micrometer scale.
Both Time-Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS) operating modes are compatible, covering a wide range of photoluminescence lifetimes, from a few ps up to seconds. Non-standard applications such as upconversion PL microscopy are possible with the appropriate choice of filters and laser coupling.
MicroPL can be configured with a computer-controlled XYZ stage for Fluorescence Lifetime Imaging Microscopy (FLIM). The FLIM add-on unlocks special features in the Fluoracle software (FluoracleMap) including control of the stage and advanced analysis options for maps, such as multi-component decay fitting algorithms. No additional software packages are required to obtain and analyse data.
| Specifications | |
|---|---|
| Microscope Models | Upright: Nikon NiU, Olympus BX53 Inverted: Nikon Ti2U |
| Excitation/Emission Range | 360 nm - 850 nm (std) Can be extended with non-standard UV and NIR objectives |
| Excitation Modes | Widefield: Tunable continuous source from spectrometer (steady state) Point: EPL/HPL/VPL pulsed lasers (TCSPC or MCS lifetimes) and CW lasers (steady state) |
| Detection Modes | Micro-PL spectroscopy with spectrometer Fluorescence Microscopy with additional camera |
| Objective Magnification | Options available from 5X to 100X |
| Sample Stage | Manual or PC-controlled XYZ stage with specifications: 75 mm x 50 mm or 130 mm x 85 mm options XY resolution 0.01 µm Z resolution 0.002 µm |
| Software | Mapping features in Fluoracle included with PC-controlled XYZ stage |
| Accessories | Software-controlled cryostat upgrade |